 Joon-hong Kim | GK |  D.C. United | 1 | 23 | 190 cm | — |
  N. Crockford | GK |  SJ Earthquakes | 22 | 23 | 193 cm | — |
 Luis Zamudio | GK | D.C. United | 56 | 28 | 191 cm | — |
 Steve Birnbaum | DF |  United States | 3 | 35 | 188 cm | — |
 Mohanad Jeahze | DF | D.C. United | 5 | 29 | 179 cm | — |
 Derek Dodson | DF |  Minnesota United FC | 18 | 27 | 185 cm | — |
 Hayden Sargis | DF | D.C. United | 30 | 24 | 183 cm | — |
 Aarón Herrera | DF |  Guatemala | — | 29 | 180 cm | — |
 Garrison Tubbs | DF | D.C. United | — | 24 | 189 cm | — |
 Boris Enow | CM | D.C. United | 6 | 26 | 180 cm | — |
 Russell Canouse | CM | D.C. United | 6 | 31 | 177 cm | — |
 Jeremy Garay | CM |  El Salvador | 8 | 23 | 175 cm | — |
 Martín Vladimir Rodríguez Torrejón | CM | D.C. United | 14 | 32 | 171 cm | — |
 Mateusz Klich | CM |  Poland | 14 | 36 | 183 cm | — |
 Theodore Ku-DiPietro | CM |  Colorado Rapids | 21 | 24 | 175 cm | — |
 Rida Zouhir | CM |  CF Montréal | 44 | 22 | 178 cm | — |
 Randall Leal | ST |  Nashville SC | 2 | 29 | 170 cm | — |
 Peglow | ST | D.C. United | 7 | 24 | 172 cm | — |
 Pedro Santos | ST | D.C. United | 7 | 38 | 173 cm | — |
 Cristian Dájome | ST | D.C. United | 11 | 32 | 172 cm | — |
 Luis Barraza | G |  Inter Miami CF | 13 | 29 | 6' 2" | — |
 Dominique Badji | ST | D.C. United | 14 | 33 | 183 cm | — |
 Gabriel Segal | F |  Houston Dynamo FC | 14 | 25 | 5' 10" | — |
 Kristian Fletcher | F |  FC Cincinnati | 14 | 21 | 6' 0" | — |
 Christian Benteke | ST |  Belgium | 20 | 35 | 190 cm | — |
| Grant Leveille | G | D.C. United | 20 | 18 | 6' 4" | — |
 Andre Dozzell | M |  Portsmouth | 21 | 27 | 5' 10" | — |
 Sean Johnson | G |  Toronto | 25 | 37 | 6' 3" | — |
| Oscar Avilez | F | D.C. United | 32 | 16 | 5' 8" | — |
 Matai Akinmboni | D |  AFC Bournemouth | 45 | 19 | 6' 3" | — |
 Fidel Junior Barajas Juárez | ST |  Real Salt Lake | 99 | 20 | 178 cm | — |
 Alex Bono | SUB |  New England | — | — | — | — |
 Hakim Karamoko | ST | D.C. United | — | 20 | 183 cm | — |
 Brandon Servania | CM- | Toronto | — | — | — | — |
 Caden Clark | SUB | Minnesota United FC | — | — | — | — |
 Christopher McVey | CD- |  San Diego FC | — | — | — | — |
 Conner Antley | SUB | D.C. United | — | — | — | — |
 David Schnegg | SUB |  Charlotte FC | — | — | — | — |
 Gabriel Pirani | SUB | San Diego FC | — | — | — | — |
 Gavin Turner | SUB | D.C. United | — | — | — | — |
 Hosei Kijima | LM |  St. Louis CITY SC | — | — | — | — |
 Jackson Hopkins | SUB | D.C. United | — | — | — | — |
 Jacob Murrell | SUB | D.C. United | — | — | — | — |
 Jared Stroud | RM | D.C. United | — | — | — | — |
 Jordan Farr | SUB | D.C. United | — | — | — | — |
| João Peglow | CF- | D.C. United | — | — | — | — |
| Keisuke Kurokawa | LB | D.C. United | — | — | — | — |
 Kye Rowles | CD- |  Australia | — | — | — | — |
 Louis Munteanu | SUB | D.C. United | — | — | — | — |
 Lucas Bartlett | CD- | D.C. United | — | — | — | — |
 Lukas MacNaughton | SUB | St. Louis CITY SC | — | — | — | — |
 Matti Peltola | CM- |  HJK | — | — | — | — |
 Nathan Ordaz | ST |  Los Angeles FC | — | 22 | 180 cm | — |
| Nikola Markovic | SUB | D.C. United | — | — | — | — |
 Sean Nealis | SUB |  New York RB | — | — | — | — |
 Silvan Hefti | RB |  Young Boys | — | — | — | — |
 Tai Baribo | CF- |  Philadelphia Union | — | — | — | — |
 Tyler Miller | SUB | Charlotte FC | — | — | — | — |